LM of HPLC column packing
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LM of HPLC column packing
Light micrograph of resin beads used in High Pressure Liquid Chromatography (HPLC), a technique for separating closely-related components from a sample solution. In this case the beads are coated with a hydophobic, waxy substance which has a long molecular backbone of 18 carbon atoms. The beads are packed into a column, usually a steel tube, through which the sample is pumped under pressure. Each component experiences an attraction to the beads which depends on its hydrophobic/hydrophilic properties, hence they emerge from the bottom of the column at different rates. Magnification: x400 at 35mm size
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Media ID 9308731
© POWER AND SYRED/SCIENCE PHOTO LIBRARY
Apparatus Chromatography Hplc Liquid Light Micrograph
EDITORS COMMENTS
This print showcases the intricate world of High Pressure Liquid Chromatography (HPLC) column packing. The image reveals a close-up view of resin beads coated with a fascinating hydophobic, waxy substance boasting an impressive molecular backbone of 18 carbon atoms. In HPLC, these meticulously packed beads play a crucial role in separating closely-related components within a sample solution. As the solution is pumped under pressure through the steel tube column containing these beads, each component experiences varying degrees of attraction based on their hydrophobic or hydrophilic properties. This results in different rates at which they emerge from the bottom of the column. The magnification level employed for this photograph is an astounding x400 at 35mm size, allowing us to marvel at the intricate details and structure of these resin beads. The vibrant colors and textures captured by Science Photo Library truly bring this scientific process to life. With its fusion of technology, apparatus, liquid chemistry, and industry expertise, HPLC has become an invaluable tool in various fields such as pharmaceuticals, environmental analysis, and food science. This print serves as a reminder of how scientific advancements continue to push boundaries and unlock new possibilities for understanding complex mixtures at a molecular level.
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